Dr. David Daleke
Associate Professor of Biochemistry and Molecular Biology
Associate Dean for Academics and Administration, University Graduate School
Dr. Daleke's interests lie in the generation and maintenance of transmembrane phospholipid asymmetry which are essential for the function of biological membranes, yet the mechanism underlying these fundamental processes remains unclear. Our research effort is designed to understand how phospholipids are assembled in biological membranes and how the resulting phospholipid asymmetry is maintained. Phospholipid transporters, or "flippases," such as the recently discovered aminophospholipid translocator, represent an interesting new class of proteins that may play a key role in the assembly and organization of phospholipids in biological membranes. These enzymes require energy in the form of ATP, have strict phospholipid structural requirements, and are unique in their ability to transport lipids across membranes. Our goal is to elucidate the structure, function, and biological significance of these proteins.
1. Shelley Cook and David L. Daleke, “Substrate Specificity of the Aminophospholipid Flippase,” in Membrane Asymmetry and Transmembrane Motion of Lipids, Philippe Devaux and Andreas Herrmann, Eds., J. Wiley & Sons, Inc., 2012, pp 199-223.
2. Magdalena Marek, Sigrid Milles, Gabriele Schreiber, David L. Daleke, Gunnar Dittmar, Andreas Herrmann, Peter Müller, and Thomas Güenther Pomorski, “The yeast plasma membrane ABC transporter Aus1: Purification, characterization and effect of lipids on its activity,” Journal of Biological Chemistry 286 (2011) 21835-21843. PMID: 21521689.
3. Jennifer A. Meyer, Wasanthi Subasinghe, Anders A. F. Sima, Zachary Keltner, Gavin E. Reid, David Daleke and Dana M. Spence, “Zinc-activated C-peptide Resistance to the Type 2 Diabetic Erythrocyte is Associated with Hyperglycemia-induced Phosphatidylserine Externalization and Reversed by Metformin,” Molecular BioSystems 5 (2009) 1157-1162. PMID: 19756305.
4. David L. Daleke, “Regulation of Phospholipid Asymmetry in the Erythrocyte Membrane,” Current Opinion in Hematology 15 (2008) 191-195. PMID: 18391783.
5. Smriti and David L. Daleke, “ATP-dependent Transport of Phosphatidylserine Analogs in Human Erythrocytes,” Biochemistry, 46 (2007) 2249-2259. PMID: 17269657.
6. David L. Daleke, “Phospholipid Flippases”, Journal of Biological Chemistry 282 (2007) 821-825 (invited review). PMID: 17130120.
7. Jill Paterson, Kathleen Renkema, Margaret Halleck, Robert Schlegel, Patrick Williamson and David L. Daleke, Lipid Specific Activation of the Murine P4-ATPase Atp8a1 (ATPase II),” Biochemistry 45 (2006) 5367-5376. PMID: 17130120.
8. Dana M. Niedowicz and David L. Daleke, “The Role of Oxidative Stress in Diabetic Complications,” Cell Biochemistry and Biophysics 43 (2005) 289-330. PMID: 16049352.
9. Peter R. Hoffmann, Jennifer A. Kench, Andrea Vondracek, Ellen Kruk, David L. Daleke, Michael Jordan, Philippa Marrack, Peter M. Henson, and Valerie A. Fadok, “Interaction between Phosphatidylserine and the Phosphatidylserine Receptor Inhibits Immune Responses In Vivo.” J Immunol 174 (2005) 1393-1404. PMID: 15661897.
- Indiana University School of Medicine Eminent Scholar, 1999
- Established Investigator, American Heart Association, 1994-1999
- Trustees Teaching Excellence Award, 1999
- American Society for Biochemistry and Molecular Biology
- American Association for the Advancement of Science
- American Chemical Society
- Biophysical Society
- American Heart Association